Proven Diagnostics Test Catalog

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ACTIVATED PROTEIN C (APC) RESISTANCE ASSAY
Test code:
 APC
Specimen type:
 Plasma
Tube type / collection container:
Specimen collection notes:
 Do not draw blood from a heparinized line. Avoid contaminating the sample with tissue thromboplastin or heparin. Venipuncture must be performed with no trauma. Hemolyzed samples are not acceptable. If blood is drawn from an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected.
What to submit:
1 mL aliquot of plasma.
Patient preparation:
 Patient should be at rest for at least 10 minutes before collecting specimen.
 
Specimen Processing Instructions:
Centrifuge the citrated blue-top tubes as soon as possible (within 60 minutes) for 15 minutes at 1500 x g to obtain platelet-poor plasma (<10,000 µL). Using a plastic pipette, remove 1 mL of plasma and transfer to a plastic aliquot tube and label with patient's name and a second patient identifier (e.g., date of birth, medical record number).
Transport temperature:
 Frozen.
Specimen stability:
 30 days frozen.
Rejection criteria:
 Clotted specimen.
 
Reference range:
 Greater than 2.1.
Alert limits:
 None.
Additional information:
 None.
CPT code(s):
 85307
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided are based on AMA guidelines and are for informational purposes only.
When performed:
 Daily, first shift
Test includes:
 APC ratio.
Methodology:
Mechanical Clot-Based
Synonyms:
APC, APC Resistance, Resistance to Activated Protein C
Clinical Significance:
The activated protein C resistance test is a clot-based screening test used to detect the possible existence of the Factor V Leiden mutation in patients. Patients with Factor V Leiden mutation are at risk for venous thrombosis.