Proven Diagnostics Test Catalog

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25OH VITAMIN D
Test code:
 25OHVD
Specimen type:
 Serum (preferred) or plasma.
Tube type / collection container:
Specimen collection notes:
 Preferred specimen is serum collected in a gold-top serum separator tube. Serum collected in a plain red-top (nonserum separator) tube is also acceptable. Plasma collected in EDTA tube or plasma separator tube is also acceptable.
What to submit:
0.5 mL serum or plasma; minimum 0.3 mL
Patient preparation:
 Fasting is preferred but not required.
 
Specimen Processing Instructions:
Allow to serum to clot. Centrifuge serum or plasma 1100-2000g for 10 minutes. Seperate from cells immediately.
Transport temperature:
 Refrigerated, 2-8°C.
Specimen stability:
 Refrigerated: 5 days. Frozen: 3 weeks.
Rejection criteria:
 Grossly hemolyzed,lipemic or samples containing particulate material. Stability limits exceeded.
 
Reference range:
 Deficient <20.0 ng/mL
Insufficent 20.0 - 29.9 ng/mL
Sufficient 30.0 - 100.0 ng/mL
High >100.0 ng/mL.
Alert limits:
 None.
Additional information:
 Vitamin D3 and Vitamin D2 are the most abundant forms of vitamin D in the body. Vitamin D3 is synthesized in the skin while D2 is derived from nutritional sources. D3 and D2 are converted to 25OH vitamin D in the liver. 25OH Vitamin is the best indicator of vitamin D nutritional status.
CPT code(s):
 82306
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided are based on AMA guidelines and are for informational purposes only.
When performed:
 Monday-Friday
Test includes:
 Total vitamin D.
Methodology:
Chemiluminescence Immunoassay
Synonyms:
Total vitamin D
Clinical Significance:
Vitamin D3 and Vitamin D2 are the most abundant forms of vitamin D in the body. Vitamin D3 is synthesized in the skin while D2 is derived from nutritional sources. D3 and D2 are converted to 25OH vitamin D in the liver. 25OH Vitamin D is the best indicator of vitamin D nutritional status.